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The selectable marker in the recombinant plasmid confers resistance to the selection and allows cells to stably integrate the plasmid into their chromosomes and grow to form foci which in turn can be cloned and expanded into cell lines
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Enzyme concentrations varied between 5 M and 0.05 M, with all Michaelis-Menton data at 0.5 M and all CML-BSA data at 5 M
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